Catalog No. | HC359066 |
---|---|
Species reactivity | Human |
Applications | ELISA, Bioactivity: FACS, Functional assay, Research in vivo |
Host species | Chimeric |
Isotype | IgG4-kappa |
Expression system | Mammalian Cells |
Clonality | Monoclonal |
Target | Leukocyte surface antigen CD47, CD47, Antigenic surface determinant protein OA3, IAP, Integrin-associated protein, Protein MER6, MER6 |
Endotoxin level | Please contact the lab for this information. |
Purity | >95% purity as determined by SDS-PAGE. |
Purification | Protein A/G purified from cell culture supernatant. |
Accession | Q08722 |
Form | Liquid |
Storage buffer | 0.01M PBS, pH 7.4. |
Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C. |
Alternate Names | AK117, AK-117, AK1172428381-55-7 |
Background | Ligufalimab (AK 117) is a humanized IgG4 anti-CD47 monoclonal antibody. Ligufalimab does not induce RBC hemagglutination, and induces phagocytosis. Ligufalimab shows anti-tumor activity. |
Note | For research use only. Not suitable for clinical or therapeutic use. |
Detects Human CD47/MER6 in indirect ELISAs.
SDS-PAGE for Research Grade Ligufalimab.
Flow-cytometry using PE anti-human CD47 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD47 monoclonal antibody (Catalog: HC359066, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using FITC anti-human CD47 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD47 monoclonal antibody (Catalog: HC359066, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using anti-human CD47 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD47 monoclonal antibody (Catalog: HC359066) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
+33(0)3 90 20 54 70
19 rue de la Haye 67300 Schiltigheim France