Research Grade Ligufalimab
Overview
Catalog No.
HC359066
Host species
Chimeric
Species reactivity
Human
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4.
Purity
>95% purity as determined by SDS-PAGE.
Clonality
Monoclonal
Isotype
IgG4-kappa
Applications
ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Target
Leukocyte surface antigen CD47, CD47, Antigenic surface determinant protein OA3, IAP, Integrin-associated protein, Protein MER6, MER6
Purification
Protein A/G purified from cell culture supernatant.
Endotoxin level
Please contact the lab for this information.
Expression system
Mammalian Cells
Accession
Q08722
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C.
Alternative name
AK117, AK-117, AK1172428381-55-7
Note
For research use only. Not suitable for clinical or therapeutic use.
Images
Bioactivity
Detects Human CD47/MER6 in indirect ELISAs.
SDS-PAGE
SDS-PAGE for Research Grade Ligufalimab.
Flow CytoMetry
Flow-cytometry using PE anti-human CD47 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD47 monoclonal antibody (Catalog: HC359066, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry
Flow-cytometry using FITC anti-human CD47 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD47 monoclonal antibody (Catalog: HC359066, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry
Flow-cytometry using anti-human CD47 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD47 monoclonal antibody (Catalog: HC359066) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.