UniProt is a comprehensive protein database collaboratively maintained by EMBL-EBI, SIB, and NCBI. It comprises UniProtKB, Proteomes, UniRef, and UniParc, offering extensive protein sequences, functional annotations, and structural data. With integrated tools like BLAST and Align, UniProt supports advanced research in bioinformatics, molecular biology, and proteomics.
Learn how to choose the best isotype control antibody to ensure accurate in vivo research. Explore essential selection criteria, their role in experimental validation, and Abinscience’s high-quality, low-endotoxin antibody solutions.
Learn about polyethylene glycol (PEG), its properties, uses, and detection using anti-PEG antibodies. Find answers to common questions on PEG-related products.
Clone numbers identify specific hybridoma cell lines in monoclonal antibody production, ensuring consistency and traceability. Find them in the 'overview' tab of the product datasheet.
This article provides a comprehensive guide to selecting and validating antibodies for life science research, emphasizing strategies to ensure experimental reliability and reproducibility. It covers matching antibodies to experimental techniques (e.g., Western Blot, ELISA, flow cytometry), understanding purification methods like affinity chromatography, and implementing robust validation approaches such as siRNA knockdown and knockout models. Practical recommendations for optimizing antibody performance are included, alongside abinScience’s commitment to providing over 15,000 rigorously validated antibodies for virology, neuroscience, and cancer research.
This article explores the structure, function, and classification of antibodies, also known as immunoglobulins, highlighting their critical role in research, diagnostics, and therapeutics. Covering heavy and light chains, Fab and Fc fragments, and the five major antibody classes (IgG, IgA, IgM, IgD, IgE), it provides insights into antigen recognition and antibody purification methods. Practical guidelines for selecting antibodies for experiments, including Western Blot, ELISA, and flow cytometry, are included, along with strategies to enhance specificity. Discover abinScience’s extensive portfolio of over 15,000 high-quality antibodies tailored for virology, neuroscience, and cancer research.
This comprehensive guide addresses common challenges in enzyme-linked immunosorbent assay (ELISA) experiments, offering practical solutions for researchers. It covers issues like low sensitivity, high background noise, non-specific binding, poor reproducibility, abnormal standard curves, color development problems, and edge effects. With detailed troubleshooting steps—such as optimizing antibody pairing, refining washing protocols, and standardizing incubation conditions—this guide empowers scientists to achieve reliable, reproducible ELISA results in immunology and biochemistry research.
Peripheral Blood Mononuclear Cells (PBMCs) are vital for immunology and oncology research, comprising T cells, B cells, NK cells, and monocytes. This standardized protocol outlines PBMC isolation via density gradient centrifugation, ensuring high-quality samples for cell culture, flow cytometry, or cryopreservation. Recommended reagents and high-performance antibodies from abinScience enhance experimental accuracy, supporting studies of immune cell subpopulations.
This guide helps researchers choose the most suitable internal control antibodies for Western Blot experiments. It outlines five key selection principles based on species, molecular weight, tissue type, expression stability, and experimental context. Practical tips and common pitfalls are included to ensure accurate and reliable protein normalization.
This article provides a comprehensive guide to troubleshooting common Western Blot (WB) issues, including high background, missing or blurry bands, and non-specific signals. Practical tips and optimization strategies are included to help researchers improve signal clarity, reduce noise, and ensure reliable results in protein detection experiments.
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