What is an Isotype Control Antibody?
In in vivo experiments, selecting and using the correct isotype control antibody is essential for accurate experimental results. These antibodies match the host species, isotype, and subclass of the primary antibody but do not bind specifically to the target antigen.
Why Are Isotype Control Antibodies Important for In Vivo Research?
They help differentiate between specific antigen-antibody interactions and non-specific binding effects. Key factors include:
- Non-Specific Binding via Fc Receptors: Monoclonal antibodies may bind non-specifically to Fc receptors (FcR), affecting results. This occurs in B cells, dendritic cells, NK cells, and macrophages.
- Host Immune Response Interference: Some monoclonal antibodies (e.g., rat or hamster IgG in mouse models) may trigger immune responses, requiring the use of a proper isotype control to mitigate this effect.
Frequently Asked Questions
- 1. Can an untreated or PBS-treated group replace an isotype control antibody?
No. Isotype control antibodies are necessary to accurately distinguish antigen-specific binding from non-specific interactions.
- 2. Is one isotype control antibody sufficient if multiple primary antibodies are used?
No. Each antibody's host species, isotype, and subclass influence its non-specific binding, so matching isotype controls must be used accordingly.
Why Choose Abinscience Isotype Control Antibodies?
- Ultra-High Purity: SDS-PAGE tested for purity, free from additional proteins or chemicals.
- Ultra-Low Endotoxin Levels: ≤ 2 EU/mg, with options for <1 EU/mg upon request.
- Low Protein Aggregation: Ensures minimal immune stimulation or experimental interference.
How to Select the Right Isotype Control Antibody?
Ensure matching host species, isotype, and subclass with the primary antibody. Abinscience provides an extensive in vivo antibody library for research needs.
Explore Our Isotype Control Antibodies
| Catalog No. |
Clone |
Product Name |
| MB107107 |
S107 |
Mouse IgA Isotype Control Antibody (S107) |
| MB107207 |
MAK |
Mouse IgA Isotype Control Antibody (MAK) |
| MV080207 |
MOPC-21 |
Mouse IgG1, kappa Isotype Control Antibody (MOPC-21) |
| MV080307 |
IS5-21F5 |
Mouse IgG1 Isotype Control Antibody (IS5-21F5) |
| MV080407 |
X40 |
Mouse IgG1, kappa Isotype Control Antibody (X40) |
