







| Catalog No. | HB769023 |
|---|---|
| Species reactivity | Human |
| Applications | ELISA, FCM, WB |
| Host species | Human |
| Isotype | IgG1, lambda |
| Clone ID | SAA2017 |
| Clonality | Monoclonal |
| Target | IL-12 subunit p40, IL12B, Interleukin-12 subunit beta, IL-12B, CLMF p40, NKSF2, NK cell stimulatory factor chain 2, Cytotoxic lymphocyte maturation factor 40 kDa subunit |
| Endotoxin level | Please contact with the lab for this information. |
| Purity | >95% as determined by SDS-PAGE. |
| Purification | Protein A/G purified from cell culture supernatant. |
| Accession | P29460 |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Note | For research use only. |

SEC-HPLC detection for Anti-Human IL12B/IL-12 p40/NKSF2 Antibody (ABS0390).

Flow-cytometry using anti-human IL12B antibody.IL12B Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL12B antibody monoclonal antibody (Catalog # HB769023 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE for Anti-Human IL12B/IL-12 p40/NKSF2 Antibody (SAA2017).

Western blot analysis was performed using anti-IL12B/IL-12 p40/NKSF2 monoclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human IL12B/IL-12 p40/NKSF2 (Catalog No: HB769021), 50ng
Lane 2: recombinant human IL12B/IL-12 p40/NKSF2 (Catalog No: HB769021), 30ng
Lane 3: recombinant human IL12B/IL-12 p40/NKSF2 (Catalog No: HB769021), 20ng




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