





| Catalog No. | HY207010 |
|---|---|
| Species reactivity | Human |
| Applications | ELISA, FCM, Neutralization |
| Host species | Human |
| Isotype | IgG1, kappa |
| Clone ID | Iv0103 |
| Clonality | Monoclonal |
| Target | Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, SPP-1, Urinary stone protein, Uropontin, SPP1, BNSP, OPN, PSEC0156 |
| Endotoxin level | Please contact with the lab for this information. |
| Purity | >95% as determined by SDS-PAGE. |
| Purification | Protein A/G purified from cell culture supernatant. |
| Accession | P10451 |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Note | For research use only. Not suitable for clinical or therapeutic use. |

Flow-cytometry using anti-human OPN antibody.HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human OPN antibody monoclonal antibody (Catalog # HY207010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE for InVivoMAb Anti-Human OPN/SPP1 (Iv0103).

Western blot analysis was performed using anti-OPN/SPP1 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human OPN/SPP1 (Catalog No: HY207012)
Lane 2: negative control




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