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Research Grade Olaratumab (HB927016)

Price(USD): $
Spec:
  • 100ug
  • 1mg
Number:
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  • Datasheet
Overview
Catalog No.HB927016
Species reactivityHuman
ApplicationsELISA, Bioactivity: FACS, Functional assay, Research in vivo
Host speciesHuman
IsotypeIgG1-kappa
Expression systemMammalian Cells
ClonalityMonoclonal
TargetPDGFR2, Platelet-derived growth factor receptor alpha, RHEPDGFRA, Alpha platelet-derived growth factor receptor, PDGFR-2, Platelet-derived growth factor alpha receptor, Platelet-derived growth factor receptor 2, PDGF-R-alpha, Alpha-type platelet-derived growth factor receptor, CD140a antigen, CD140 antigen-like family member A, PDGFRA, PDGFR-alpha, CD140a
Endotoxin levelPlease contact the lab for this information.
Purity>95% purity as determined by SDS-PAGE.
PurificationProtein A/G purified from cell culture supernatant.
AccessionP16234
FormLiquid
Storage buffer0.01M PBS, pH 7.4.
Stability and StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C.
Alternate Names3G3, IMC-3G3, LY3012207, 1024603-93-7
BackgroundOlaratumab (IMC-3G3; LY3012207) is an anti-platelet-derived growth factor receptor alpha (PDGFRα) human monoclonal IgG1 antibody with antitumor activity.
NoteFor research use only. Not suitable for clinical or therapeutic use.
Images
  • SEC-HPLC

    SEC-HPLC detection for Research Grade Olaratumab.

  • Bioactivity

    Detects CD140a/PDGFRA in indirect ELISAs.

  • Flow Cytometry

    Flow-cytometry using anti-human PDGFRA antibody. MG-63 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human PDGFRA monoclonal antibody (Catalog HB927016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • SDS-PAGE

    SDS-PAGE for Research Grade Olaratumab.

  • Flow CytoMetry

    Flow-cytometry using FITC anti-human CD140a antibody. MG-63 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD140a monoclonal antibody (Catalog: HB927016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

  • Flow CytoMetry

    Flow-cytometry using PE anti-human CD140a antibody. MG-63 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD140a monoclonal antibody (Catalog: HB927016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

  • Flow CytoMetry

    Flow-cytometry using APC anti-human CD140a antibody. MG-63 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD140a monoclonal antibody (Catalog: HB927016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

References

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