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Research Grade Alemtuzumab (HB894016)

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  • 100ug
  • 1mg
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Overview
Catalog No.HB894016
Species reactivityHuman
ApplicationsELISA, Bioactivity: FACS, Functional assay, Research in vivo
Host speciesHumanized
IsotypeIgG1-kappa
Expression systemMammalian Cells
ClonalityMonoclonal
TargetHuman epididymis-specific protein 5, CDW52, CAMPATH-1 antigen, Cambridge pathology 1 antigen, He5, HE5, Epididymal secretory protein E5, CD52, CDw52
Endotoxin levelPlease contact the lab for this information.
Purity>95% purity as determined by SDS-PAGE.
PurificationProtein A/G purified from cell culture supernatant.
AccessionP31358
FormLiquid
Storage buffer0.01M PBS, pH 7.4.
Stability and StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C.
Alternate NamesCampath-1H, LDP-03, 216503-57-0
BackgroundAlemtuzumab (Campath-IH) is a humanized monoclonal antibody against CD52. Alemtuzumab does not cross-react with murine CD52. Alemtuzumab selectively targets the CD52 antigen to induce profound lymphocyte depletion, followed by recovery of T and B cells with regulatory phenotypes. Alemtuzumab is capable of complement-dependent cytotoxicity and antibody-dependent cell-mediated cytotoxicity (ADCC), as well as induction of apoptosis. Alemtuzumab has the potential for B-cell chronic lymphocytic leukaemia research.
• Changes of the major sperm maturation-associated epididymal protein HE5 (CD52) on human ejaculated spermatozoa during incubation., PMID:11420384
• Interaction of the human epididymal protein CD52 (HE5) with epididymal spermatozoa from men and cynomolgus monkeys., PMID:9291477
• Vasectomy influences expression of HE1 but not HE2 and HE5 genes in human epididymis., PMID:14662784
• A major mRNA of the human epididymal principal cells, HE5, encodes the leucocyte differentiation CDw52 antigen peptide backbone., PMID:8418821
• Function of human epididymal proteins in sperm maturation., PMID:9739419
• Epididymal epithelium immortalized by simian virus 40 large T antigen: a model to study epididymal gene expression., PMID:11574662
• Body temperature (37 C) specifically down-regulates the messenger ribonucleic acid for the major sperm surface antigen CD52 in epididymal cell culture., PMID:8828507
NoteFor research use only. Not suitable for clinical or therapeutic use.
Images
  • Bioactivity

    SEC-HPLC detection for Research Grade Alemtuzumab.

  • SDS-PAGE

    SDS-PAGE for Research Grade Alemtuzumab.

  • Flow Cytometry

    Flow-cytometry using anti-human CD52 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD52 monoclonal antibody (Catalog HB894016) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.

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