| Catalog No. | YP809016 |
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| Species reactivity | General |
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| Applications | ELISA, Bioactivity: FACS, Functional assay, Research in vivo |
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| Host species | Mouse |
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| Isotype | IgG1-kappa |
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| Expression system | Mammalian Cells |
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| Clonality | Monoclonal |
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| Target | Idiotope of anti-(N-glycolylneuraminic acid (NeuGc, NGNA) -gangliosides GM3) Mus musculus IgM-kappa monoclonal antibody P3/chP3 |
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| Endotoxin level | Please contact the lab for this information. |
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| Purity | >95% purity as determined by SDS-PAGE. |
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| Purification | Protein A/G purified from cell culture supernatant. |
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| Form | Liquid |
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| Storage buffer | 0.01M PBS, pH 7.4. |
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| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C. |
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| Alternate Names | 1E10, 946832-34-4 |
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| Background | Adenosine 3', 5'-cyclic monophosphate (cAMP) is a key second messenger molecule in intracellular signal transduction. Monitoring cAMP levels is one of the most common ways to screen for agonists and antagonists of G protein–coupled receptors(GPCRs). Bioyeargene cAMP ELISA Kit is based on the competition between HRP-labeled cAMP and free cAMP for fixed amounts of mouse monoclonal anti-cAMP antibody(cAMP MAb) binding sites. Because the concentration of the HRP-labeled cAMP is held constant while the concentration of cAMP varies, the amount of HRP-labeled cAMP that is able to bind to the cAMP MAb will be inversely proportional to the concentration of cAMP in the well. This antigen-antibody complex binds to the goat anti-mouse IgG that has been previously attached to the well. The plate is washed to remove any unbound reagents and then developed. The ELISA typically displays an IC50(50% B/B0) of approximately 12 pmol/mL and a detection limit of approximately 0.15 pmol/mL.
• Sphingosine 1-phosphate: Lipid signaling in pathology and therapy., PMID:31624181
• Sphingosine-1-phosphate (S1P) in ovarian physiology and disease., PMID:31455516
• Sphingosine-1-phosphate (S1P) receptors: Promising drug targets for treating bone-related diseases., PMID:32155312
• Pancreatic Acinar Cells-Derived Sphingosine-1-Phosphate Contributes to Fibrosis of Chronic Pancreatitis via Inducing Autophagy and Activation of Pancreatic Stellate Cells., PMID:37634735
• Significance of sphingosine-1-phosphate in cardiovascular physiology and pathology., PMID:32278039
• Sphingosine 1-Phosphate (S1P)/ S1P Receptor Signaling and Mechanotransduction: Implications for Intrinsic Tissue Repair/Regeneration., PMID:31703256
• Sphingosine 1-phosphate (S1P) receptor modulators as an induction and maintenance therapy for ulcerative colitis: a systematic review and meta-analysis of randomized controlled trials., PMID:38153524
• S1P (Sphingosine-1-Phosphate)-Induced Vasodilation in Human Resistance Arterioles During Health and Disease., PMID:36070401
• Sphingosine 1-phosphate (S1P) signalling: Role in bone biology and potential therapeutic target for bone repair., PMID:28855094
• Sphingosine 1-phosphate signalling., PMID:24346695
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| Note | For research use only. Not suitable for clinical or therapeutic use. |
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