Anti-Human CD228/MELTF Antibody (sc57.43) (HY094107)

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Overview

Catalog No.	HY094107
Species reactivity	Human
Applications	FCM
Host species	Mouse
Isotype	IgG1, kappa
Clone ID	sc57.43
Clonality	Monoclonal
Target	CD228, Melanotransferrin, Melanoma-associated antigen p97, MAP97, MFI2, MELTF
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P08582
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Note	For research use only.

Images

Bioactivity

Detects CD228/MELTF in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human CD228 antibody.A375 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD228 antibody monoclonal antibody (Catalog # HY094107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human CD228 antibody.A431 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD228 antibody monoclonal antibody (Catalog # HY094107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet