| Catalog No. | VK425014 |
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| Species reactivity | Chikungunya virus, O'nyong-nyong virus |
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| Applications | WB, IHC, ELISA |
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| Host species | Rabbit |
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| Isotype | IgG |
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| Species | Chikungunya virus (strain S27-African prototype) (CHIKV) |
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| Clonality | Polyclonal |
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| Immunogen | E. coli - derived recombinant CHIKV Structural polyprotein (Tyr810-Lys1221). |
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| Tested applications | WB, ELISA |
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| Target | CHIKV-E1 |
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| Endotoxin level | Please contact with the lab for this information. |
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| Purity | >90% as determined by SDS-PAGE. |
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| Purification | Purified by antigen affinity column. |
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| Accession | Q8JUX5, Q5WQY5, Q5XXP3, O90371, P22056, O90369 |
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| Form | Liquid |
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| Storage buffer | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. |
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| Product Usage Information |
| Application |
Dilution |
| ELISA |
1:5000-1:20000 |
| IHC |
1:50-1:500 |
| WB |
1:500-1:2000 |
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| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
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| Alternate Names | Structural polyprotein, p130, Capsid protein, 3.4.21.90, Coat protein, C, Precursor of protein E3/E2, p62, pE2, Assembly protein E3, Spike glycoprotein E2, E2 envelope glycoprotein, 6K protein, Spike glycoprotein E1, E1 envelope glycoprotein |
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| Background | Chikungunya virus (CHIKV) is a mosquito-borne alphavirus responsible for Chikungunya fever, a disease marked by fever, rash, and joint pain. The E1 envelope glycoprotein, a 47 kDa class II fusion protein, plays a critical role in viral entry by mediating low pH-triggered membrane fusion within host endosomes. It forms heterodimers with E2 glycoproteins, assembling into spike trimers on the viral surface that facilitate host cell attachment and infection. Due to its essential function in viral infectivity, the E1 protein is widely used in studies of viral entry, antibody development, vaccine design, and diagnostic assay optimization.
• Vertebrate-class-specific binding modes of the alphavirus receptor MXRA8., PMID:37804831
• Changes in the chikungunya virus E1 glycoprotein domain II and hinge influence E2 conformation, infectivity, and virus-receptor interactions., PMID:38842335
• Two Conserved Phenylalanine Residues in the E1 Fusion Loop of Alphaviruses Are Essential for Viral Infectivity., PMID:35416719
• The chikungunya virus E1 glycoprotein fusion loop and hinge alter glycoprotein dynamics leading to cell and host specific changes in infectivity., PMID:37961096
• Construction and Evaluation of Recombinant Adenovirus Candidate Vaccines for Chikungunya Virus., PMID:36016401
• Enhanced Molecular Surveillance of Chikungunya Virus., PMID:31270168
• Cell-based analysis of Chikungunya virus E1 protein in membrane fusion., PMID:22520648
• Promising application of monoclonal antibody against chikungunya virus E1-antigen across genotypes in immunochromatographic rapid diagnostic tests., PMID:32615978
• Expression of plasmid-based shRNA against the E1 and nsP1 genes effectively silenced Chikungunya virus replication., PMID:23056297
• A vaccine candidate based on baculovirus displaying chikungunya virus E1-E2 envelope confers protection against challenge in mice., PMID:39440961
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| Note | For research use only. |
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