DOWNLOAD DATASHEET
Anti-PIGR Polyclonal Antibody
Overview
Catalog No. HF948014
Host species Rabbit
Species reactivity Capra hircus, Human
Immunogen E. coli - derived recombinant Human PIGR (Arg289-Arg638).
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Clonality Polyclonal
Isotype IgG
Applications ELISA, IHC, WB
Target Poly-Ig receptor, Polymeric immunoglobulin receptor, PIgR, PIGR, Hepatocellular carcinoma-associated protein TB6
Purification Purified by antigen affinity column.
Accession P01833
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Product Usage Information
ApplicationDilution
ELISA1:5000-1:20000
IHC1:50-1:500
WB1:500-1:2000
Note For research use only.
Images


Immunofluorescence
Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting PIGR (HF948014) at a concentration of 16 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of PIGR was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).

Western blot
Western blot analysis was performed using anti-PIGR polyclonal antibody at 1μg/ml on various samples.
Lane 1: K562 cell lysate
Lane 2: HL60 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: MOLT-4 cell lysate