| Catalog No. | HW776207 |
| Host species | Mouse |
| Species reactivity | Human |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4, 0.09% Sodium azide. |
| Purity | >95% as determined by SDS-PAGE. |
| Clonality | Monoclonal |
| Isotype | IgG2a, kappa |
| Applications | ELISA, FCM |
| Target | BU63, B70, CD28LG2, CTLA-4 counter-receptor B7.2, Activation B7-2 antigen, T-lymphocyte activation antigen CD86, CD86, FUN-1 |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | Please contact with the lab for this information. |
| Accession | P42081 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Clone ID | SAA1397 |
| Note | For research use only. |
Anti-Human CD86/B7-2 Antibody (SAA1397)
Overview
Images
 Bioactivity |
Detects Human CD86/B7-2 in indirect ELISAs. |
 Flow Cytometry |
Flow-cytometry using anti-human CD86 antibody.Daudi cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD86 antibody monoclonal antibody (Catalog # HW776207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 Flow Cytometry |
Flow-cytometry using anti-human CD86 antibody.K562 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD86 antibody monoclonal antibody (Catalog # HW776207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer. |