Anti-Human NECTIN4/PVRL4 Antibody (SAA0403)

Catalog No.	HV985207
Host species	Mouse
Species reactivity	Human
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 0.09% Sodium azide.
Purity	>95% as determined by SDS-PAGE.
Clonality	Monoclonal
Isotype	IgG2a, kappa
Applications	ELISA, FCM
Target	LNIR, NECTIN4, PRR4 receptor-related protein 4, Nectin cell adhesion molecule 4, Ig superfamily receptor LNIR, Nectin-4, PVRL4
Purification	Protein A/G purified from cell culture supernatant.
Endotoxin level	Please contact with the lab for this information.
Accession	Q96NY8
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID	SAA0403
Note	For research use only.

Bioactivity	Detects NECTIN4/PVRL4 in indirect ELISAs.
Flow Cytometry	Flow-cytometry using anti-human NECTIN4 antibody.NECTIN4 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human NECTIN4 antibody monoclonal antibody (Catalog # HV985207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry	Flow-cytometry using anti-human NECTIN4 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human NECTIN4 monoclonal antibody (Catalog: HV985207) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Western blot	Western blot analysis was performed using anti-NECTIN4 monoclonal antibody at 1ug/mL on various samples. Lane 1: recombinant human NECTIN4 (Catalog No: HV985011) Lane 2: negative control