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Anti-Human CD324/CDH1 Antibody (67A4)
Overview
Catalog No. HY460107
Host species Mouse
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS, pH 7.4.
Purity >95% as determined by SDS-PAGE.
Clonality Monoclonal
Isotype IgG1, kappa
Applications FCM, IF, IP
Target CD324, Uvomorulin, Epithelial cadherin, E-cadherin, CDHE, CAM 120/80, UVO, CDH1, Cadherin-1
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level Please contact with the lab for this information.
Accession P12830
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID67A4
Note For research use only.
Images


Bioactivity		 Detects CD324/CDH1 in indirect ELISAs.

Flow Cytometry		 Flow-cytometry using anti-human CD324 antibody.HT-29 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD324 antibody monoclonal antibody (Catalog # HY460107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow Cytometry		 Flow-cytometry using anti-human CD324 antibody.MCF7 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD324 antibody monoclonal antibody (Catalog # HY460107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow CytoMetry		 Flow-cytometry using anti-human CD324 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD324 monoclonal antibody (Catalog: HY460107) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.