DOWNLOAD DATASHEET
Anti-Human IgE/IGHE Antibody (SAA0343)
Overview
Catalog No. HV064033
Host species Mouse
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS, pH 7.4.
Clonality Monoclonal
Isotype IgG2a, kappa
Applications ELISA, FCM
Target Immunoglobulin heavy constant epsilon, Ig epsilon chain C region, Ig epsilon chain C region ND, IGHE
Purification Protein A/G purified from cell culture supernatant.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone IDSAA0343
Note For research use only.
Images


Flow Cytometry
Flow-cytometry using anti-human IgE antibody.IgE Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IgE antibody monoclonal antibody (Catalog # HV064033 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow Cytometry
Flow-cytometry using anti-human IgE antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human IgE antibody monoclonal antibody (Catalog # HV064033 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE
SDS-PAGE for Anti-Human IgE/IGHE Antibody (SAA0343).