Anti-Human CFH Antibody (SAA0527)
Overview
Catalog No. |
HY505013 |
Host species |
Mouse |
Species reactivity |
Human |
Form |
Liquid |
Storage buffer |
0.01M PBS, pH 7.4. |
Purity |
>95% as determined by SDS-PAGE. |
Clonality |
Monoclonal |
Isotype |
IgG2a, kappa |
Applications |
ELISA, FCM, WB |
Target |
Complement factor H, CFH, H factor 1, HF1, HF, HF2 |
Purification |
Protein A/G purified from cell culture supernatant. |
Endotoxin level |
Please contact with the lab for this information. |
Accession |
P08603 |
Stability and Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
Clone ID | SAA0527 |
Note |
For research use only. |
Images
 Flow Cytometry |
Flow-cytometry using anti-human CFH antibody.HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CFH antibody monoclonal antibody (Catalog # HY505013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer. |
 SDS-PAGE |
SDS-PAGE for Anti-Human CFH Antibody (SAA0527). |
 Flow CytoMetry |
Flow-cytometry using PE anti-human CFH antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CFH monoclonal antibody (Catalog: HY505013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer. |
 Flow CytoMetry |
Flow-cytometry using FITC anti-human CFH antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CFH monoclonal antibody (Catalog: HY505013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer. |
 Flow CytoMetry |
Flow-cytometry using APC anti-human CFH antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CFH monoclonal antibody (Catalog: HY505013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer. |