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Anti-Human CFH Antibody (SAA0527)
Overview
Catalog No. HY505013
Host species Mouse
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS, pH 7.4.
Purity >95% as determined by SDS-PAGE.
Clonality Monoclonal
Isotype IgG2a, kappa
Applications ELISA, FCM, WB
Target Complement factor H, CFH, H factor 1, HF1, HF, HF2
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level Please contact with the lab for this information.
Accession P08603
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone IDSAA0527
Note For research use only.
Images


Flow Cytometry
Flow-cytometry using anti-human CFH antibody.HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CFH antibody monoclonal antibody (Catalog # HY505013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE
SDS-PAGE for Anti-Human CFH Antibody (SAA0527).

Flow CytoMetry
Flow-cytometry using PE anti-human CFH antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CFH monoclonal antibody (Catalog: HY505013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Flow CytoMetry
Flow-cytometry using FITC anti-human CFH antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CFH monoclonal antibody (Catalog: HY505013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Flow CytoMetry
Flow-cytometry using APC anti-human CFH antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CFH monoclonal antibody (Catalog: HY505013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.