Research Grade Sacituzumab
Overview
Catalog No.
HY373016
Host species
Humanized
Species reactivity
Human
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4.
Purity
>98% purity as determined by SDS-PAGE.
Clonality
Monoclonal
Isotype
IgG1-kappa
Applications
ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Target
Pancreatic carcinoma marker protein GA733-1, Tumor-associated calcium signal transducer 2, M1S1, TROP2, TACSTD2, Cell surface glycoprotein Trop-2, GA733-1, Membrane component chromosome 1 surface marker 1
Purification
Protein A/G purified from cell culture supernatant.
Endotoxin level
Please contact the lab for this information.
Expression system
Mammalian Cells
Accession
P09758
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C.
Alternative name
IMMU-132, hRS7-SN-38, hRS7-SN-38-ADC, hRS7-[CL-SN-38], 1796566-95-4
Note
For research use only. Not suitable for clinical or therapeutic use.
Images
SEC-HPLC
SEC-HPLC detection for Research Grade Sacituzumab.
Bioactivity
Detects Human TACSTD2/TROP2 in direct ELISAs.
SDS-PAGE
SDS-PAGE for Research Grade Sacituzumab.
Flow Cytometry
Flow-cytometry using anti-human TACSTD2 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human TACSTD2 monoclonal antibody (Catalog HY373016) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry
Flow-cytometry using anti-human TACSTD2 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human TACSTD2 monoclonal antibody (Catalog HY373016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry
Flow-cytometry using anti-human TACSTD2 antibody. MCF-7 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human TACSTD2 monoclonal antibody (Catalog HY373016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry
Flow-cytometry using anti-human TACSTD2 antibody. MDA-MB-468 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human TACSTD2 monoclonal antibody (Catalog HY373016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry
Flow-cytometry using anti-human TACSTD2 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human TACSTD2 monoclonal antibody (Catalog: HY373016) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.