Anti-Human MTHFD2 Polyclonal Antibody
Overview
Catalog No. |
HB832014 |
Host species |
Rabbit |
Species reactivity |
Human, Mouse, Bos taurus, Chicken |
Immunogen |
E. coli - derived recombinant Human MTHFD2 (Glu36-Asn350). |
Form |
Liquid |
Storage buffer |
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. |
Clonality |
Polyclonal |
Isotype |
IgG |
Applications |
ELISA, IHC, WB |
Target |
NMDMC, MTHFD2, Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial |
Purification |
Purified by antigen affinity column. |
Accession |
P13995 |
Stability and Storage |
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
Product Usage Information |
Application | Dilution |
---|
ELISA | 1:5000-1:20000 | IHC | 1:50-1:500 | WB | 1:500-1:2000 |
|
Note |
For research use only. |
Images
 Immunofluorescence |
Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting MTHFD2 (HB832014) at a concentration of 10.4 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of MTHFD2 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue). |